Fig. 4 | Scientific Reports

Fig. 4

From: An investigation into the mechanism for Kaempferol improving melanocyte death based on network Pharmacology and experimental verification

Fig. 4

Kae interfered with the ferroptosis of HEM-1. (A) Damage to HEM-1 by different concentrations of RSL3. (B) Protective effects of Kae on the ferroptosis of HEM-1. (C) The number of HEM-1 in each group under a microscope. (D) Mitochondrial morphological changes of HEM-1 treated with RSL3, Kae, and their combination for 24 h. (E) Changes in the membrane potential in HEM-1 treated by RSL3, Kae, and their combination for 24 h. (F) The production of intracellular ROS was detected by FC. (G) The production of lipid ROS was detected by FC. (H) Changes in ferroptosis in HEM-1 treated with RSL3 and Kae. (I) The accumulation of ferroptosis in cells was detected by fluorescence microscopy. (J) WB analysis of GPX4, a key protein of ferroptosis. (K) Activities of antioxidant enzymes (GSH Px, SOD, and CAT).

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