Fig. 5
Production of disulfide bond-containing NMDA receptor agonist binding domains in BB. (a) Schematic representing disulfide bonds (solid lines connecting pink bars denoting C420-C454, C436-C455, C744-C798) for GluN1 and GluN2A agonist binding domains (ABDs) as seen in previous structural alignment34. (b) Cartoon representation of GluN1/GluN2A ABD heterodimer with glutamate bound to GluN2A and 7-chlorokynurenic acid (7-CKA) bound to GluN1. (c) Chemical structures of competitive GluN1 antagonists, 5,7-dichlorokynurenic acid (DCKA) and 7-CKA. (d) Ribbon diagram of GluN1 (purple)/GluN2A (green) ABD heterodimer in complex with 7-CKA and glutamate (PDB 9DA9) rotated to show ligand-binding pockets. (e) Top: schematics of GluN2A/GluN1 ABD heterodimers bound to DCKA/glutamate (red and blue, left) or to 7-CKA/glutamate (grey and purple, right). Bottom: An overlay of DCKA/glutamate bound GluN2A/GluN1 ABD heterodimer (PDB: 4NF4) with 7-CKA/glutamate bound GluN2A/GluN1 ABD heterodimer (PDB 9DA9). Insets: zoomed in agonist binding pockets with bound ligands (also overlaid).
