Fig. 2
Genomic distribution of DMRs in the rumen of dairy buffaloes fed green forage or standard diet. (a) Chromosomal distribution of the DMRs identified in the Treated as compared with the Control group. Chromosomal length is displayed on the x axis (Mb). Chromosomes are sorted by size. Red bars indicate hypermethylated DMRs, while green bars show hypomethylated DMRs. The presence of multiple DMRs in the same genomic region is indicated by longer bars. The percentage of DMRs in each chromosome is reported on the right of the figure. (b) and (c) Scatterplots that show the correlation of the number of DMRs with the number of genes on the different chromosomes (b) or with the chromosome size (c). The Pearson’s correlation coefficients and the statistical significance are displayed. (d) Left: Percentage of DMRs localized in intergenic and genic (exons, introns and promoters) regions. Right: Number of hypomethylated and hypermethylated DMRs included in intergenic regions, exons, introns and promoters. For promoters, DMRs located up to 2 kb upstream of the transcriptional start site is considered. (e) Pie chart showing the distribution of the DMGs according to their biotype.
