Fig. 7

AAV-IKV Mediated Gene Delivery to the Non Human Primate Cornea. (A) GFP expression in the eyes of live African green monkey (Chlorocebus sabaeus) at baseline, day 14 and day 37 post intracameral injection of AAV-IKV-GFP + Nuc1 in the oculus dexter (OD, right eye) and oculus sinister (OS, left eye). Whereas there was no detectable GFP fluorescence prior to injection (baseline), at day 14 post injection there was clear evidence of GFP expression in the cornea in a punctate pattern of expression (e.g. arrowheads) in live animals. Images containing arrowheads are higher magnification of the boxed areas. At all time points, the cornea of both eyes exhibited transparency and lack of any significant signs of inflammation, suggesting that the research grade vector did not lead to any significant immune responses. (B) Frozen cryosections examined for expression of GFP at day 45 post injection revealed GFP-positive cells in the cornea. (C) Intraocular pressure of AAV-IKV-GFP + Nuc1 injected eyes was similar to baseline at day 14 and 37 post injection. (D) High resolution OCT imaging of cornea, sclera and anterior chamber angles of AAV-IKV-GFP injected eyes at day 14 and day 37 were similar to baseline (B). Volume scans revealed an average thickness of the retina at day 14 and 37 was similar to baseline (C). High resolution OCT imaging of retina of AAV-IKV-GFP injected eyes at day 37 was also similar to baseline. See Supplementary Fig. 2 for day 14.