Table 1 Primers used in vector construction.

From: Structural analysis and core promoter prediction of STAR gene and its regulatory mechanism of progesterone synthesis in bovine luteal cells

Gene name

Primer sequence(5’-3’)

Annealing

Temperature/℃

Product

Length/bp

Primer Usage

STAR-F

STAR-R

atttgcggccgcGCCACCATGCTTCTCGCGACGTTTAAGCT

ccggaattcTCAACACCTAGCTTCAAGAGCAGG

63

63

858

CDS region amplification

STAR-1 F

gaagatctCAGGTAGACAGCAAAGGGACTCAGT

64

472

Different deletion fragments

Amplification

STAR-2 F

gaagatctAGAATGGTCTGGAGTGAGTGAGGTTG

64

1003

STAR-3 F

gaagatctAATGTGCGCTTGAGTGTCGGTAC

64

1499

STAR-4 F

gaagatctTAGAGTGCTCAGGGCATCTGCTG

64

1990

STAR-R

ctagctagcTCACGGTGGCTCAGTGGCAAAGAA

66

  
  1. Note: The Italicized characters indicate the cleavage site and protection base; The Underscores indicate cleavage sites, The Bold letters are the protective bases of restriction enzymes.
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