Table 2 Primers used in RT-qPCR.
Gene name | Primer sequence(5’-3’) | Annealing Temperature/℃ | Product Length/bp | NCBI Accession NO. |
|---|---|---|---|---|
STAR | F: GACGTTTAAGCTGTGTGCCG R: TTGGTCGCTGTAGAGAGGGT | 60 | 202 | NM_174189.3 |
3βHSD | F: GAGGAAATGGGGGTGCTAGA R: AACAGCAGCTGGGTACCTTTC | 60 | 239 | NM_174343.3 |
CYP11A1 | F: AGAGAATCCACTTTCGCCACT R: GTAGGATCCCTCGAACTTGGA | 60 | 197 | XM_010809903.3 |
GAPDH | F: GTACATGGTCTACATGTTCCAG R: GGTTGTGGATGGTAGTGAAGA | 60 | 200 | NM_001034034.2 |
