Fig. 1
From: ZIP13 marks muscle satellite cells and contributes to their quiescent and active phase balance
Properties of MuSCs in Zip13-KO mice. (A) mRNA expressions of MRFs in the skeletal muscle of Zip13-KO mice. (B) Cell populations of ITGA7 positive MuSCs. The cells derived from hind legs of either WT or Zip13-KO mice were analyzed using FACS. The ITGA7 positive MuSCs were gated based on the fluorescent intensity derived from the fluorescent-labeled isotype control antibody. (C) The number of ITGA7-positive MuSCs of WT and Zip13-KO mice were normalized using either muscle mass or the number of viable cells. (D) mRNA expressions of Zip13 and MuRFs in ITGA7-positive MuSCs as shown in (B). (E) The FACS analysis of the active and quiescent MuSCs of either WT or Zip13-KO mice. The ITGA7 and CD34 positive populations were gated based on the fluorescent intensity derived from the fluorescent-labeled isotype control for either ITGA7 or CD34 antibodies. A ration of the quiescent- and active-phase MuSCs gated as purple and blue squares (dot plots), respectively, were quantified (bar graphs). Student’s t-test was used to compare the two groups. Values are means ± S.E. of 6 mice. The asterisk indicates a significant difference (*p < 0.05).
