Fig. 4
From: ZIP13 marks muscle satellite cells and contributes to their quiescent and active phase balance
The effect of ZIP13 on the regeneration of the skeletal muscle tissues in an acute muscle injury model. (A) Hematoxylin and eosin (H&E) staining of muscle tissue sections from BaCl2-injected WT and Zip13-KO mice. White arrow indicates the nuclei around the muscle fibers. Black arrow indicates nuclei localization into the center of the muscle fibers. Higher magnification data surrounded in black squares in each H&E staining panel are presented below in each panel. The quantitative analysis of H&E staining was conducted. The perimeter (upper right) and cross sectional area (lower right) of the muscle fibers on Day 0 and Day 10 in both WT or Zip13-KO mice were shown as box-plot graphs. The Cohen’s d values were also calculated and shown beneath each box-plot graphs. (B) Gene expression analysis of the skeletal muscle of either WT or Zip13-KO mice in a muscle injury model. The samples were collected on days 0, 5, and 10 after the BaCl2 injection. Student’s t-test was used to compare the two groups. The asterisk indicated a significant difference (*p < 0.05, **p < 0.01). Values are means ± S.E. of 3–5 mice. (C) GFP and Tomato fluorescent intensity of quiescent and activated phases of MuSCs, respectively were monitored using FACS and expressed as histogram. The MuSCs of Zip13-GFP/Tomato-KI mice on days 0, 20, and 30 after BaCl2 injection were subjected to FACS analysis.
