Fig. 7
RRT extract restores inflammatory factors induced HFF senescence. (a) Representative images of β-Gal staining (magnification: ×20). (b) Flow cytometry analysis of β-Gal activity in HFF. (c) Relative COL3A1 mRNA levels in HFF. (d) Western blot electrophoresis results and relative COL1A1, TGF-β1, and Smad2/3 proteins levels in HFF. (e) The relative mRNA expression levels of SASP factors, including IL-6, IL-1α, MMP1, MMP3, CXCL1, and TGF-β1, in HFF. Statistical analysis involved one-way ANOVA and paired two-tailed t-tests, with significance denoted as ###P < 0.001, ##P < 0.01, and #P < 0.05 relative to the control group, and ***P < 0.001, **P < 0.01, and *P < 0.05 relative to the inflammation medium group.
