Fig. 2

STAM2 is upregulated in BCa cells and tissues. (A-B) STAM2 mRNA and protein levels were detected using qRT‒PCR and western blotting in tumor tissues and adjacent normal tissues. C Quantitative western blot analysis of the data in B. (D-E) STAM2 mRNA and protein levels were detected using qRT‒PCR and western blotting in an epithelial cell line (SV-HUC-1) and BCa cell lines (UM-UC-3, T24, and 5637). (F) Quantitative western blot analysis of E. (All PVDF bands were transferred and tested in the same western blot experiments, and the experiments performed in triplicate.)