Fig. 2
From: Random mechanisms govern bacterial succession in bioinoculated beet plants
Bacterial communities and sets of PICRUSt2-predicted functions encoded in their genomes (predicted functional potential) in soils, roots, and leaves differ in terms of structure, alpha diversity, and bacterial load. L—leaves, R—roots, S—soils, I—inoculant (lyophilized wild-growing sea beet roots). (A) NMDS analysis of the d05 generalized UniFrac distance matrix; black circles -inoculant (lyophilized wild sea beet roots); blue diamonds—soils; green squares—roots; red-filled circles—leaves. Results of a PERMANOVA and betadisper tests of a model involving Material (a factor with levels of ‘inoculant’, ‘soil’, ‘root’, and ‘leaf’) are presented on the graph. (B) Alpha diversity of ASVs. Boxplots of Shannon’s diversity index (H’)—upper left, Shannon’s evenness—upper right, richness (observed number of ASVs) – lower left, number of bacterial 16S rRNA gene sequences per ng of DNA—lower right. (C) dbRDA of the Morisita-Horn distance matrix derived from predicted functions sets imputed by PICRUSt2; (D) alpha diversity of predicted functions. Boxplots of Shannon’s diversity index (H’) (upper left), Shannon’s evenness (upper right), and richness (observed number of predicted functions) (lower left). (E) Ternary plot of ASVs displaying differential abundance in soils, roots, and leaves. The mean ASV abundance is conveyed as a circle area, and the colour denotes the classification at the class level: Alphaproteobacteria, red; Bacilli, green; Bacteroidia, blue; and Gammaproteobacteria, purple. (F) Ternary plot of predicted KO functions displaying differential abundance in soils, roots and leaves; the scale is arbitrary. Colours: antibiotic resistance and biosynthesis—red, carbohydrate metabolism—yellow‒green, heavy metal resistance—green, quorum sensing—blue, and secretion systems—purple. (G) Taxonomic structure of the soil (S), root (R), and leaf (L) communities at the genus level; the genera identified by DESeq2 and ALDEx2 as significantly more abundant in a specific material are marked with a letter denoting the compartment: s—soil, r—root, l—leaf.
