Table 2 Primer and probe sequences for the detection of HPS.

From: Establishment and validation of a simple and accurate qPCR detection method for Haemophilus parasuis

Primer/Probe

Sequences(5’- 3’)

Product length(bp)

HPS-F1

GCTTTCGATAATGCGACGTGCT

161 bp

HPS-R1

TGCCGTTGAAAGCTCGTGTAAAGA

HPS-P1

VIC- TACCACCTACGCCAGAGCCAACC -TAMRA

HPS-F2

GCCTGAAAGTCCTAATACTTCCACA

286 bp

HPS-R2

GGCTTCAAGTAAGTCGTCAATCCCCATT

HPS-P2

VIC- GCACTTAATTCTAATACTTCCGATTGA -MGB

β-actin-F

TGCACAGTAGGTCTGACGTG

88 bp31

β-actin-R

CCAGACTGGGGACATGCAG

β-actin-P

FAM-TCCCCAGCACACTTAGCCGTGTT-BHQ1

  1. HPS-F1/R1/P1 refers to the initial set of primer probes designed for HPS detection, while HPS-F2/R2/P2 denotes the second set of primer probes for the same purpose. The β-actin-F/R/P primer probe is specifically designed for the porcine housekeeping gene β-actin.
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