Fig. 4

Mutation profile of the VP1 region sequenced by Nanopore and Sanger directly from the wastewater specimen WW22-4017. (A) Nucleotide and (B) amino acid differences as compared to Sabin 2 (AY184220.1) are depicted. Whole genome sequence data of WW22-4017 poliovirus isolate (PQ641085) is included for comparative purposes. Differences highlighted in red are the common ones shared with USA-NY-22. Sanger_WW (PQ641087), Nanopore_rep1 (PQ641089) and Nanopore_rep2 (PQ641090). WW = wastewater.