Fig. 2

The expression of Ca handling proteins in left ventricular tissue samples. Representative western blots showing SERCA2 (a), annexin 6A (b) phospholamban (c, top), phospho (Ser16/Thr17)-phospholamban (c, bottom) and sarcolipin (d, monomer and multimer formation) protein levels in left ventricular tissue, which was obtained from wt, DMD^mdx and IVA-treated DMD^mdx rats. This figure also shows the same lanes after membranes were re-probed for GAPDH or vinculin to demonstrate equal protein loading in each lane. Semi-quantitative analysis of SERCA2 (e), annexin 6A (f) phospholamban (g) and phospho (Ser16/Thr17)- phospholamban (h), phospho (Ser16/Thr17)- phospholamban/phospholamban (i) and sarcolipin (j–l) in left ventricular tissue. Data represent means ± SEM; n = 8 for wt, n = 6 for DMD^mdx, and n = 6 for IVA-treated DMD^mdx. Statistical comparisons were carried out using a one-way ANOVA and post-hoc Tukey’s multiple comparison test, **p < 0.01. DMD Duchenne Muscular Dystrophy, GAPDH glyceraldehyde 3-phosphate dehydrogenase, IVA ivabradine, PLN phospholamban, p-PLN phospho (Ser16/Thr17)-phospholamban, SERCA2 sarcoplasmic/endoplasmic reticulum Ca ATPase 2, ns not significant, wt wild-type.