Fig. 1 | Scientific Reports

Fig. 1

From: Rigid enlargement of sybodies with antibody fragments for cryo-EM analyses of small membrane proteins

Fig. 1

Scheme showing the NabFab and Legobody assemblies. In both, the Fabs (heavy chain, HC, in dark blue and light chain, LC, in light blue) bind to the backside of modified sybodies. For the NabFab assembly, the Fab binds to a sybody from the loop library (light green) harboring an amino acid change in its backbone or a sybody where the CDRs are grafted onto the TC-Nb4 scaffold. For the Legobody assembly, the Fab binds directly to sybodies (light green) containing an amino acid change in the backbone. The assemblies are completed with a Fab-binding nanobody (Anti-Fab Nb, green) in the case of NabFab, or with a sybody- and Fab-binding MBP_PrA/G fusion protein (green) in the case of Legobody.

Back to article page