Fig. 2 | Scientific Reports

Fig. 2

From: Rigid enlargement of sybodies with antibody fragments for cryo-EM analyses of small membrane proteins

Fig. 2

Sybody binding by the Legobody Fab. (A) Binding of sybodies with different termini by the Legobody Fab measured using GCI. (B) Affinity determination of the Legobody Fab binding to three sybodies stemming from three distinct sub-libraries (concave: Sb#1520, loop: Syb3719 and convex: Sb#6820). All these sybodies contain the C-terminus (iv) shown in (A). Measured data (red curve) was fitted with a Langmuir 1:1 binding model (black curve) and values for on-rate (ka), off-rate (kd) and dissociation constant (KD) are given in the respective graphs.

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