Fig. 5 | Scientific Reports

Fig. 5

From: Rigid enlargement of sybodies with antibody fragments for cryo-EM analyses of small membrane proteins

Fig. 5

GCI measurement using loop-grafted sybodies. (A) Affinity determination of Sb#15, Sb#45 and Sb#68 and their grafted versions onto TC-Nb4 against immobilized SARS-CoV-2 spike protein. (B) Affinity determination of Sb_MBP#1 and its grafted version onto TC-Nb4 against immobilized MBP. Measured data (red curve) was fitted with a Langmuir 1:1 binding model (black curve) and values for on-rate (ka), off-rate (kd) and dissociation constant (KD) are given in the respective graphs.

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