Fig. 1
From: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2
Daidzein inhibits TGF-β1‐induced proliferation and migration of mouse primary dermal fibroblasts. (A) CCK-8 analysis cell toxicity of Dai in DFs (n = 3); (B) CCK-8 analysis cell viability of Dai in DFs (n = 3); (C) Representative images and quantitative results of EdU incorporation assay in DFs (n = 5) (40×, scale bar = 20 μm); (D) Representative images and quantitative analysis of wound healing assay in DFs (n = 5) (20×, scale bar = 200 μm); (E) Representative images and quantitative results of migration in DFs (n = 5) (20×, scale bar = 200 μm); (F) The mRNA levels of MMP-1, MMP-2 and MMP-13 in DFs (n = 5). The data are shown as Mean ± SD, one-way ANOVA with Tukey’s post-hoc multiple comparison tests. ####, p < 0.0001 vs. CTL; **, p < 0.01, ***, p < 0.001, ****, p < 0.0001 vs. TGF-β1. CTL, Control; Dai, Daidzein; DFs, Mouse primary dermal fibroblasts.
