Fig. 3
From: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2
Daidzein inhibits TGF-β1‐induced activation of mouse primary dermal fibroblasts. (A) The mRNA levels of α-SMA, Col-1 and Fn in DFs (n = 5); (B) The protein levels of α-SMA, Col-1 and Fn in DFs (n = 5); (C) The protein levels of p-Smad2, t-Smad2, p-Smad3, t-Smad3 and GAPDH in DFs (n = 5); (D) Immunofluorescence representative images and quantitative results of α-SMA in DFs (n = 5) (40×, scale bar = 20 μm); (E) Immunofluorescence representative images and quantitative results of Col-1 in DFs (n = 5) (40×, scale bar = 20 μm); (F) Immunofluorescence representative images and quantitative results of p-Smad3 in DFs (n = 5) (40×, scale bar = 20 μm). The data are shown as Mean ± SD, one-way ANOVA with Tukey’s post-hoc multiple comparison tests. ####, p < 0.0001 vs. CTL; *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001 vs. TGF-β1. CTL, Control; Dai, Daidzein; DFs, Mouse primary dermal fibroblasts; Fn, Fibronectin; Col-1, Collagen-I.
