Fig. 6
Acquisition of a fixed Hela cell labelled with WGA 594 immersed in water during its flow through the patterned light sheet. (a–c) Raw images of a single plane for different phase shifts. (d) Fourier transform of the acquired fluorescence signal. (e) Cross-correlation of the image in frequency space. (f) Comparison between the expected and measured phases that were applied. (g) Widefield (WF) and reconstructed SIM image of the plane shown in a–c. (h) Detail of (g) highlighted by the green box, showing the resolution improvement after SIM reconstruction (bottom panel) with respect to the WF (top panel). (i) Intensity profiles retrieved along the horizontal lines (x direction) shown in (h), highlighting the improvement obtained both in resolution and contrast with the SIM reconstruction. (j) Selected single planes, equally spaced of 1.1 \(\upmu {\rm m}\), acquired during the cell flow through the patterned light-sheet in WF (top panel) and SIM (bottom panel) modality. (k) View of the x-z plane of the cell acquired in (j), showing the improvement of the reconstructed SIM (right panel) with respect to the WF case (left panel). Scale bar is always 5 \(\upmu {\rm m}\).
