Fig. 1 | Scientific Reports

Fig. 1

From: Potential upscaling protocol establishment and wound healing bioactivity screening of exosomes isolated from canine adipose-derived mesenchymal stem cells

Fig. 1

Characterization of cAD-MSCs. (A, B) Morphology representation of isolated cAD-MSCs was observed using phase-contrast microscopy at 40X and 200X magnifications. (C) Stemness markers (Oct4 and Rex1) and proliferative marker (Ki67) mRNA expression were assessed via RT-qPCR and normalized to a reference gene (Gapdh). (D) Expression of MSC and hematopoietic-related surface markers was analyzed using Flow cytometry. (E) Osteogenic differentiation potency was identified by Alizarin red staining and analysis of gene expression for osteogenic-related markers. (F) Chondrogenic differential potency was determined using Alcian blue staining and chondrogenic-related gene expression analysis. (G) Adipogenic differential potency was evaluated through Oil Red O staining and the mRNA expression of adipogenic-related markers. mRNA expression of multilineage differentiation markers was normalized to the reference gene and the undifferentiated group served as control. The mean and p-value are depicted, with asterisks denoting the significance levels (*p < 0.05, **p < 0.01, ***p < 0.001).

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