Fig. 2 | Scientific Reports

Fig. 2

From: Potential upscaling protocol establishment and wound healing bioactivity screening of exosomes isolated from canine adipose-derived mesenchymal stem cells

Fig. 2

Establishment of culture medium potential for cAD-MSc exosome collection. (A) Schematic of experimental design. (B) Morphological representation of cAD-MSCs cultured with various exosome-collecting media investigated at days 1, 2, 3, and 4 of incubation. (C) Live/dead staining of cAD-MSCs upon the culture within various exosome-collecting media. Live cells were labeled with green fluorescence using calcein-AM, while dead cells were marked with red fluorescence using propidium iodide. (D) The viability of cAD-MSCs cultured in different exosome-collecting media was evaluated using the resazurin assay on days 1, 2, 3, and 4. The results are presented as fold change normalized to the control group for each day of incubation. The mean and p-value are denoted (n = 4), with asterisks presenting the significance levels compared to the control (*p < 0.05, **p < 0.01, ***p < 0.001). The scale bar indicates 250 μm.

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