Fig. 4 | Scientific Reports

Fig. 4

From: Potential upscaling protocol establishment and wound healing bioactivity screening of exosomes isolated from canine adipose-derived mesenchymal stem cells

Fig. 4

Quantitative and productivity assessment of cAD-MSC-derived exosome production protocols. (A) Schematic workflow of exosome production. (B) Production yield comparison, calculated by total particle production analyzed from NTA divided by initial cell seeding number in various production protocols (n = 4). (C) Comparison of exosome concentration (particle/mL) in conditioned medium of cell cultured using different production protocol (n = 4). (D) The ratio of particle number to microgram protein obtained from different exosome production protocols. The mean and p-value are noted. (E) Pearson’s correlation coefficient between the particle-to-protein ratio and CD9 positivity, obtained from exosome characterization across all groups (n = 9). R-value and p-value are plotted. The asterisks indicate the significance levels (*p < 0.05, **p < 0.01, ***p < 0.001).

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