Fig. 5
From: Biochemical and physiological characterization of Aedes aegypti midgut chymotrypsin
Ae. aegypti midgut blood protein digestion extract analysis. (A) Representative protein gels of FLUC and RNAi AaCHYMO midgut extracts collected at 3 h to 72 h PBF (0.006 midgut equivalents were loaded onto a NuPAGE 4–12% BIS-TRIS gel (Invitrogen)). The protein gels shown in the figure were cropped for clarity and combined for direct comparison. The original unaltered protein gels are presented in Supplementary File 1. MW represents the pre-stained PageRuler protein ladder in kilodaltons (kDa) (Thermo Scientific #26616). (B) Column plot comparing the pixel area of the protein species running at the 70 kDa MW marker (purple arrow in A) between RNAi AaCHYMO and FLUC midgut extract samples. (C) Column plot comparing the pixel area of the protein species running between the 50 and 70 kDa MW marker (red arrow in A) between RNAi AaCHYMO and FLUC midgut extract samples. D. Column plot comparing the pixel area of the protein species running at the 15 kDa MW marker (teal arrow in A) between RNAi AaCHYMO and FLUC midgut extract samples.
