Fig. 6

AaCHYMO is predicted to interact with IgG and less likely to interact with human albumin or hemoglobin. Modeled structure of AaCHYMO by AlphaFold2 (gray) was submitted to HDOCK to predict potential interactions with (A) human serum albumin (PDB ID: 1E7H, slate blue), (B) human hemoglobin (PDB ID: 1GZX, salmon), and (C) human neutralizing IgG (PDB ID: 1HZH, cyan). Predictions suggest the latter’s potential to interact and have residues close to the catalytic site of the enzyme (~ 7Å). Results suggest that the two other human proteins can potentially engage, but inconclusive for full cleavage to occur. Predicted interactions are shown as black dashed lines, while measured distances to the catalytic serine are shown as yellow dashed lines. Heme groups are displayed as sticks (brown). Catalytic residues are displayed as sticks (gray) and labeled (H48, D97, and S193). Structures are represented by cartoons and a transparent surface. All images were generated with PyMOL software (v2.5.7).