Fig. 4
From: Melatonin-protein interactions are dependent on yeast carbon metabolism
Shifting metabolism from respiration to fermentation. Yeast cells were grown first in respiratory conditions (YPE), and after 48 h, 5% (w/v) of glucose were added to shift the metabolism to fermentation. Growth kinetics determined by monitoring yeast growth (measured as OD600) and the time course of proteins bound to melatonin in (A) S. cerevisiae CLOS, (B) S. cerevisiae QA23, and (C) L. thermotolerans LAKTIA. Full-length images of the gels are provided as Supplementary material.
