Fig. 5

The expression level of cholinergic marker proteins in HT-22 cells during the treatment of agarwood ethanol extract. (A) Bar graph representing the amount of choline and acetylcholine content in HT-22 cells treated with AWE and compared to control cells; (n = 4) (B) Representative western-blot images of ChAT and muscarinic acetylcholine receptor (mAChR/M2) in HT-22 cells. (C) The graph represents the semi-quantified data using Image J. The data are obtained from three independent experiments and expressed as the means ± SEM (n = 3). One-Way ANOVA, followed by Dunnett’s post hoc test, evaluated the statistical differences. *p < 0.05, **p < 0.01, compared with control group.