Fig. 1

Experimental design of prenatal exposure to BPA. (A) Diagram showing exposure timelines and procedures. (B) Adult male and female mice were allowed to mate at a 2:1 female to male ratio. Pregnant mice were exposed with BPA (50 mg/kg/day) via intragastric injection from embryonic day (ED) 0.5 to 18.5. The male offspring mice were anatomized at postnatal day (PND) 70. The diagrams show experimental workflow of this study.The figure was made by using Biorender system (www.biorender.com).