Fig. 7

Apoptosis detection by TUNEL assay and expression of BAX/BCL-2 of control and prenatal BPA-exposed offspring testes. (A) TUNEL assay in control and prenatal BPA-exposed offspring testes. The blue staining was the nucleus stained by DAPI, and the green signals were the apoptotic cells. (B) Western blot analysis of BAX/BCL-2 in control and prenatal BPA-exposed offspring testes with triple replications. Each bar represented 50 μm. The data were analyzed by t-test. p < 0.05 was considered significance (Supplemental Fig. 1) .