Fig. 3 | Scientific Reports

Fig. 3

From: Interactions of juvenile hormone, 20-hydroxyecdysone, developmental genes, and miRNAs during pupal development in Apis mellifera

Fig. 3

Validation of the predicted binding sites for miR-34 and miR-281 in the 3′UTR of the genes EcR, Kr-h1, Chd64, InR-2 and ftz-f1, by luciferase assay. Changes in the ratio of chimeric Renilla luciferase (Ren) and firefly luciferase (FF) activity is shown for each gene. The chimeric Ren luciferase is linked to the 3′UTR of target genes containing the predicted binding sites for miR-34 and miR-281. Schematic representation of the positive control, perfect pairing with the miR-34 and miR-281 sequence (A and F). The positive control (B and G) and the 3′UTRs of the Chd64 (B), InR2 (C), Kr-h1 (D) ftz-f1 (H), and EcR (I) genes were directly regulated by miR-281. (J) Network of interactions based on the validated binding sites between the miR-34 and miR-281, in the 3′UTR of the target genes: EcR, Kr-h1, Chd64, InR-2 and ftz-f1. Bars show means + -SEM (n = 6) T-test: * < 0.05.

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