Fig. 3
From: Factor H-related 2 levels dictate FHR dimer composition
Insights into FHR-1 and -2 dimerization. (a) FHR-1/2 ELISA was used to show the rapid formation of FHR-1/2 dimers over time when incubating equimolar amount of FHR-1 and FHR-2 at 37 °C. A plasma pool deficient in FHR-1 or FHR-2 was used as source for FHR-2/2 and FHR-1/1 respectively. FHR-1/2 levels were measured at 4 °C to halt further dimer formation. (b) Impact of pH on the stability of FHR-1 and -2 dimers. Normal human serum (NHS, 3% v/v) was incubated at the indicated pH using an isotonic 40 mM BisTrispropane buffer. pH 8.5 was used as reference, resembling the pH of the in-house dimer ELISAs. (c) Impact of sodium chloride on dimer stability. NHS (3%, (v/v) was incubated with increasing concentrations of sodium chloride ranging from 0.0625–2 M. 125 mM NaCl was used as a 100% reference. Symbols represent mean of three separate measurements with error bars indicating the SD.
