Table 2 Calculation table used for calibrating FHR-1/2 ELISA.
From: Factor H-related 2 levels dictate FHR dimer composition
Sample | % Pool 1 (%, v/v) | % Pool 2 (%, v/v) | FHR-1/1 measured in sample | FHR-2/2 measured in sample | Formed FHR-1/2 (theoretical) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Start | After | Delta (Δ) | Start | After | Delta (Δ) | Based on ΔFHR-1/1 | Based on ΔFHR-2/2 | Average | |||
Pool 1: FHR-2 def. | – | – | 273.84 | - | – | 0.00 | – | – | – | – | – |
Pool 2: CFHR3/CFHR1 def. | – | – | 0.00 | - | – | 81.20 | – | – | – | – | – |
Mix A | 95 | 5 | 260.15 | 245.57 | − 14.58 | 4.06 | 0.51 | − 3.55 | 29.16 | 7.11 | 18.13 |
Mix B | 85 | 15 | 232.76 | 213.50 | − 19.26 | 12.18 | 2.10 | − 10.08 | 38.52 | 20.17 | 29.35 |
Mix C | 75 | 25 | 205.38 | 187.34 | − 18.04 | 20.30 | 4.50 | − 15.80 | 36.08 | 31.60 | 33.84 |
Mix D | 50 | 50 | 136.92 | 115.99 | − 20.93 | 40.60 | 14.16 | − 26.43 | 41.86 | 52.87 | 47.36 |
Mix E | 25 | 75 | 68.46 | 47.64 | − 20.82 | 60.90 | 34.15 | − 26.75 | 41.65 | 53.49 | 47.57 |
Mix F | 15 | 85 | 41.08 | 24.81 | − 16.27 | 69.02 | 47.17 | − 21.85 | 32.53 | 43.69 | 38.11 |
Mix G | 5 | 95 | 13.69 | 4.37 | − 9.32 | 77.14 | 66.73 | − 10.41 | 18.65 | 20.81 | 19.73 |
