Fig. 5
From: RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer
Time to threshold for samples before and after gain optimization. The time to threshold for third eyelids from white-tailed deer which tested positive by real-time quaking-induced conversion assay before (red circles) and after (green squares) gain optimization on the plate reader used to run the assay. Figure 5a: The optimized gain adjustment significantly reduced the time to threshold from an average of 28.1 (dotted red line) to 20.3 (dashed green line) hours for individual samples (paired T-test, t = 7.6, p = 0.00003, df = 9. Figure 5b: The optimized gain adjustment significantly reduced the time to threshold from an average of 37.4 (dotted red line) to 28.3 (dashed green line) for the 30 pools of 10 samples that contained at least one third eyelid from a deer diagnosed with CWD based on ELISA/IHC results from lymph nodes and/or obex (paired Wilcoxon signed-rank test, V = 398, p = 0.0003).
