Table 1 Overall sensitivity and specificity for CWD prion detection in individual third eyelids using RT-QuIC.

From: RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer

ELISA/IHC results from RPLN/obex

Wild

Captive

Total

Third eyelid

RT-QuIC positive

Third eyelid

RT-QuIC negative

Se/Sp

CWD-positive

96

25

121

114 (TP)

7 (FN)

Se 94%

CWD-negative

119

10

129

0 (FP)

129(TN)

Sp 100%

Total

215

35

250

   
  1. The overall sensitivity (Se) and specificity (Sp) of real-time quaking-induced conversion (RT-QuIC) on third eyelids compared to previous disease-status for that individual white-tailed deer determined by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) of retropharyngeal lymph nodes (RPLN) and/or obex of wild and captive white-tailed deer (n = 250). TP = true positive. FN = false negative. FP = false positive. TN = true negative.
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