Table 3 Sensitivity and specificity for CWD prion detection in pools containing 5 third eyelids using RT-QuIC.

From: RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer

Number of third eyelids from CWD-positive deer

RT-QuIC positive

RT-QuIC negative

Se/Sp

4

7 (TP)

0 (FN)

Se 100%

2

7 (TP)

0 (FN)

Se 100%

1

7 (TP)

0 (FN)

Se 100%

0

0 (FP)

11 (TN)

Sp 100%

Overall

21 (TP), 0 (FP)

0 (FN), 11 (TN)

Se 100%, Sp 100%

  1. The sensitivity (Se) and specificity (Sp) of real-time quaking-induced conversion (RT-QuIC) on pools of 10 third eyelids (2) or 5 third eyelids (3) from white-tailed deer compared to previous disease-status determined by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) of retropharyngeal lymph nodes and/or obex. The column on the far left indicates the number of third eyelids in the pool that were from white-tailed deer with lymph nodes which had previously tested positive by ELISA and IHC. TP = true positive. FN = false negative. FP = false positive. TN = true negative.
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