Fig. 1
From: Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle
Centriolar localization of centrobin during the cell cycle (a) HeLa cells at G1 phase were prepared with the mitotic shake-off method, and synchronously cultured for up to 16 h to reach to the S phase. (b) HeLa cells at S phase were prepared with the double thymidine block and release method, and cultured for up to 8 h to reach to the M phase. (c) HeLa cells at M phase were enriched with the thymidine-RO3306 release method. Mitotic stages of the individual cells were determined with the DAPI staining. (a–c) The cells at indicated time points were coimmunostained with centrin2, centrobin and Cep164 antibodies. Representative images of the staining patterns are shown in Supplementary Fig. 1. The number of centriole signals per cell was counted. (d) The cells were arrested at G1/S transition phase with the double thymidine block method, and synchronously released for 2, 6 and 8 h to reach to S, G2 and G1 phases, respectively. The cells were coimmunostained with centrobin (green) and Cep164 (red) antibodies. Scale bar, 10 μm. (e) The number of cells with centrobin signals at young and old mother centrioles was counted. Statistical significance was determined using paired t-test. *, P < 0.05. (a-c, e) More than 90 cells per group were counted in 3 independent experiments. (f) Summary of the centriolar localization of centrobin during the cell cycle.
