Fig. 2

Precocious installation of DAs in the centrobin^KO centrioles (a) The Tp53^KO;centrobin^KO HeLa cells were arrested at G1 and M phases, and coimmunostained with centrin2 (red) and centrobin (green) antibodies. (b) Several lines of the Tp53^KO;centrobin^KO HeLa cells were subjected to immunoblot analyses with centrobin and Gapdh antibodies. (c) The Tp53^KO;centrobin^KO HeLa cells at G1 and M phases were coimmunostained with the centrin2 (green) and Cep164 (red) antibodies. (d) The number of centriolar Cep164 signals per cell was counted in the Tp53^KO;centrobin^KO HeLa cells. (e) The centrobin^KO and TP53^KO;centrobin^KO RPE1 cells were coimmunostained with centrin2 (red) and centrobin (green) antibodies. (f) Several lines of the centrobin^KO and Tp53^KO;centrobin^KO RPE1 cells were subjected to immunoblot analyses with centrobin and Gapdh antibodies. (g) The centrobin^KO RPE1 cells at G1 and M phases were coimmunostained with centrin2 (green) and Cep83 (red) antibodies. (h) The number of centriolar Cep83 signals per cell was counted in the centrobin^KO RPE1 cells. (a, c,e, g) Scale bars, 10 μm. (d, h) More than 90 cells per group were counted in 3 independent experiments. Statistical significance was determined using paired t-test. *, P < 0.05.