Fig. 5
From: Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle
Plk1 regulation of centrobin localization at mitosis (a) Plk1-depleted HeLa cells were arrested at S, G2, and M phases, and coimmunostained with centrobin (green) and Cep164 (red) antibodies. (b) The number of centrobin signals per cell was counted at indicated cell cycle stages. (c) The number of cells with centrobin signals at young and old mother centrioles was quantified. (d) HeLa cells at S, G2, and M phases were treated with BI2536 for 6 h, and coimmunostained with centrobin (green) and Cep164 (red) antibodies. (e) The number of centrobin signals were counted in cells at indicated cell cycle stages. (f) The number of cells with centrobin signals at young and old mother centrioles was quantified. (a, d) Scale bars, 10 μm. (b, c,e, f) More than 90 cells per group were counted in 3 independent experiments. Statistical significance was determined using paired t-test. *, P < 0.05; **, P < 0.01.
