Fig. 6
From: Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle
Plk1 phosphorylation of centrobin for its localization at mitosis Tp53KO;centrobinKO HeLa cells were stably rescued with the wild type (GFP-centrobin) and Plk1-phospho-resistant mutant centrobin (GFP-CbnPlk1R). (a, b) The cells were arrested at S, G2, and M phases and coimmunostained with centrobin and Cep164 antibodies. The number of centrobin (a) and Cep164 (b) signals per cell was counted. (c) The cells were arrested at G1/S transition phase with the double thymidine block method, and synchronously released for 2 and 6 h to reach to S and G2 phases, respectively. The cells were coimmunostained with centrobin (green) and Cep164 (red). Scale bar, 10 μm. (d) The number of cells with centrobin signals at young and old mother centrioles was counted. (a, b, d) More than 90 cells per group were counted in 3 independent experiments. Statistical significance was determined using paired t-test. *, P < 0.05; **, P < 0.01.
