Fig. 7
From: Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle
Nek2 phosphorylation of centrobin for its localization at interphase Tp53KO;centrobinKO HeLa cells were stably rescued with the wild type (GFP- Cbn), Nek2-phospho-resistant mutant centrobin (GFP-CbnNek2R), and Plk1-phospho-resistant mutant centrobin (GFP-CbnPlk1R). (a) Mitotic cells were isolated using the shake-off method and re-plated to allow synchronous entry into the G1 phase. (b) The cells were arrested at G1/S transition phase with the double thymidine block method and synchronously released to S phase. (a, b) The cells were collected at 2-hour intervals and coimmunostained with centrin2, centrobin, and Cep164 antibodies. The numbers of centrin2, centrobin, and Cep164 signals per cell were counted at each time point. More than 90 cells per group were counted in 3 independent experiments.
