Fig. 1
From: The translational inhibitor 4EBP/Thor is required for Drosophila adaptation to hypoxia
Thor transcriptional induction during hypoxia is dependent on Sima and Foxo. (a) X-Gal staining was assessed in normoxia (Nx, 21% O2) and hypoxia (Hx) in the Thor-enhancer-trap fly line (Thor-lacZ) in germ band-extended embryos, 3rd instar larval fat body, and adult indirect flight muscle (3% O2, 5 h for embryos and larvae; 4% O2 5 h for adults). (b) Control (w1118), sima homozygous mutant (simaKG07607) or foxo mutant (foxo21/25) embryos were maintained in normoxia (Nx) or exposed to 3% oxygen for 5 h (Hx), and Thor mRNA levels were assessed by qRT-PCR using RPL29 as an internal control. Data are shown as the mean + standard deviation of three independent experiments with three replicates each. Two-way ANOVA, *p < 0.05; **p < 0.001; n = 3. (c) Thor-enhancer-trap activity was determined in Thor-lacZ embryos overexpressing Sima with an en-Gal4 driver (en-Gal4; UAS-sima) in comparison to control (en-Gal4) embryos.
