Fig. 2
From: Toward real-time margin assessment in breast-conserving surgery with hyperspectral imaging

Orientation of the lumpectomy specimen during data acquisition. The specimen is considered as a cube with six resection sides. To acquire hyperspectral images of the entire resection surface, each side of the specimen is imaged according to a fixed order (sides 1-6). Surgical sutures, placed during the procedure to preserve the specimen’s original orientation, serve as reference markers. A single suture indicates the side facing the nipple, while double sutures indicate the side facing the dorsal (back) of the patient. These sutures allow for cross-referencing the imaged resection sides with the pathology results. Based on the suture markings, the specimen was inked and processed in the pathology department, where the surgical margin status of each side was evaluated by a pathologist.