Fig. 2
LncRNA FTX promotes the proliferation and glycometabolism of CRC cells in vitro. (A) LncRNA FTX expression in HT29 cells transfected with shRNAs targeting lncRNA FTX and in HCT116 cells following lncRNA FTX overexpression via RT-qPCR. (B) Cell viability was determined using CCK-8 assay after transfection of sh-FTX and oe-FTX. (C) Cell migration and invasion was detected using Transwell assay after transfection of sh-FTX and oe-FTX. (D) Enzymatic activity levels of LDH and Lactate production were determined using corresponding assay kits when HT29 and HCT116 cells were transfected. (E) Protein expression levels of HK2, PKM2 and LDHA by western blot. The mean and standard error of the mean of three independent experiments performed in triplicate. *p < 0.05. Data are expressed as mean ± standard deviation; t-test was used to analyze data. HK2, Hexokinase 2; PKM2, Pyruvate kinase isozyme type M2; LDHA, Lactate dehydrogenase A. sh, short hairpin. CS, citrate synthase; IDH1, isocitrate dehydrogenase 1.
