Fig. 4

LncRNA FTX sequester miR-215-3p to enhance colorectal cancer cell proliferation and aerobic glycolysis. (A) The potential binding site between lncRNA FTX and miR-215-3p predicted by Starbase. (B) Pearson’s correlation analysis was performed to determine the correlation between lncRNA FTX and miR-215-3p expression. (C–D) miR-215-3p expression levels in FTX-depleted HT29 cells (C), and (D) FTX-overexpressing in HCT116 cells, in the corresponding control cells determined by RT-qPCR. (E) The targeting relationship between lncRNA FTX and miR-215-3p was confirmed by dual-luciferase reporter assay. (F–G) Growth inhibition evaluated by CCK-8 (F) and colony formation (G) assays in the indicated cells. (H) Transwell invasion assay of the cells described in. (I) Western blot analyses were performed to determine YAP1 and c-Myc expression after miR-215-3p upregulation in HCT116 cells overexpressing lncRNA FTX. *p < 0.05. Data are expressed as mean ± SD; t-test or ANOVA was used to analyze data.