Fig. 5
Intranasal immunization. (A) Immunization schedule. (B) CVB1 neutralizing antibodies, (C) Vaccine antigen-specific IgA and (D) IgG antibody end point titers in bronchoalveolar lavage at week 6 after two intranasal immunizations. (E) IFN-γ, (F) IL-2 and (G) TNFα secreting splenocytes after stimulation with CVB1. (H) CVB1 neutralizing antibodies, (I) antigen-specific IgG, (J) IgG1 and (K) IgG2a antibodies from termination serum. Antigen specific antibodies were determined with indirect ELISA. Titers below 200 were assigned as negative. Neutralizing antibodies determined with microneutralization assay. Titers below 8 were assigned as negative. Shown as scatter dot plots with geometric mean and geometric SD. Cytokine secreting splenocytes were determined with FluoroSpot assay and shown as spot-forming units (SFU)/106 splenocytes, mean with SD. * = p < 0.05, ** = p < 0.01 *** = p < 0.001, **** = p < 0.0001.
