Fig. 6
CryoEM data collection and image processing. Panels A-I are related to CVB1-VLPTween80 dataset and J-R to CVB1formalin dataset. (A) and (J) Representative micrograph from each dataset. Scale bar is shown on the figure. An example of picked particles is boxed in white with a corresponding box size. (B) and (K) Representative 2D classes after final 2D classification of particles chosen for further processing obtained by Relion 4 in Scipion (B) or cryoSPARC (K). Scale bar of 30 nm is shown. (C) and (L) 3D classes obtained by Relion 4 in Scipion (C) or heterogeneous refinement in cryoSPARC (L). Particles in classes boxed in blue were chosen for further processing together within each dataset as they correspond to compact particles. Particles contributing to class boxed in red correspond to expanded particles. Symmetry axes are marked with elipse (2-fold), triangle (3-fold) and pentagon (5-fold). Scale bar of 30 nm is shown on the bottom figure. (D, E,M, N) Fourier shell correlation (FSC) curves for compact (D) and expanded (E) CVB1-VLPTween80 and compact (M) and expanded (N) CVB1formalin final reconstructions obtained by non-uniform refinement in cryoSPARC v4.2.0 (VLP) and v4.4.1 (formalin inactivated)31. The global resolution of CVB1-VLPTween80 structures is estimated at 2.15 Å as the estimation reaches the Nyquist limit. The legend for FSC curves calculated with different masks applied is on the right. (F, G,O, P) Central plane views of the final reconstructions for compact (F, O) in blue and expanded (G, P) in red. Symmetry axes are marked with elipse (2-fold), triangle (3-fold) and pentagon (5-fold). Scale bars of 15 nm are shown below the figures. (H, I, Q, R) Local resolution of final reconstructions for compact (H, Q) in blue and expanded (I, R) in red shown as a central slice of surface representation at 2.5σ above mean (H, I, Q) or 5.5 σ above mean (R). Local resolution values legend is shown in Ångstrom. Symmetry axes are marked with elipse (2-fold), triangle (3-fold) and pentagon (5-fold).
