Fig. 3
Power to identify relatedness of strains between infections is enhanced by highly multiplexed microhaplotypes. Simulated infections using population allele frequencies from available WGS data were used to estimate the power of testing if a pair of strains between infections is related. Countries in each of three continents with the most available WGS data were selected. Infections were simulated for a range of COI. Only one pair of strains between the infections was related with a given expected IBD proportion (r). The results were compared for reconstructed microhaplotypes and their most highly variable SNP for 3 panels (MAD4HatTeR, SpotMalaria and AMPLseq). Note that SpotMalaria bioinformatics pipeline outputs a 100 SNP barcode, and thus its actual power (dark orange) is not reflective of the potential power afforded by microhaplotypes (light orange). Additionally, the 50 most diverse microhaplotypes and their corresponding SNPs were used to evaluate the effect of down-sizing MAD4HatTeR (MAD4HatTeR50).
