Fig. 4 | Scientific Reports

Fig. 4

From: Sustainable production of osteoinductive Co2+, Mg2+ and Mn2+ -substituted apatites particles by one-pot conversion of biogenic calcium carbonate

Fig. 4

Interaction of 25 µg/mL particles with m17.ASC and mOBPs cells analyzed by TEM and flow cytometry after 24 h of exposure. Representative TEM photomicrographs show particles internalization in m17.ASC (a) and mOBPs (d) cells compared to untreated controls (CTRL−). Photomicrographs are representative of alternate serial cuts of the cell pellets of each sample. Scale bar: 2–5 µm. Representative dot plots show changes in morphology, based on physical parameters such as size (FSC-A) and complexity (SSC-A), in m17.ASC (b) and mOBPs (e) cells treated with particles, compared to untreated controls (CTRL−). Representative histograms display the percentage of cells with increased complexity, categorized by low/medium or high SSC-A for m17.ASC (c) and mOBPs (f) cells relative to the untreated control (CTRL−).

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