Fig. 7 | Scientific Reports

Fig. 7

From: Sustainable production of osteoinductive Co2+, Mg2+ and Mn2+ -substituted apatites particles by one-pot conversion of biogenic calcium carbonate

Fig. 7

Effect of the particles on the activity of ALP enzyme on m17.ASC and on mOBPs cells. Optical microscope images of the stained m17.ASC (a) and mOBPs cells (c), magnification 200×. Quantitative analysis of ALP activity on m17.ASC (b) and mOBPs cells (d) obtained by counting stained area with ImageJ software, statistic was calculated using one-way ANOVA with Dunnett’s comparison post-test versus CTRL− (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

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