Fig. 8

Effect of the particles on calcium phosphate deposits in the cell matrix of m.17.ASC and mOBPs cells stained with ARS and quantified. Optical microscope images of the stained m17.ASC (a) and mOBPs cells (c), magnification 200×. Quantitative analysis of ARS obtained by dissolving calcium phosphates deposits of m17.ASC (b) and mOBPs cells (d), and the absorbance was then measured. Statistics were calculated using one-way ANOVA with Dunnett’s comparison post-test vs. CTRL− (****p < 0.0001).